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KMID : 0387420080190030179
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Korean Society of Oriental Neuropsychiatry
2008 Volume.19 No. 3 p.179 ~ p.193
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The Effects of Antioxidant and Anti-Alzheimer on Hydrogen peroxide and β-amyloid peptid-induced PC 12 cells by Semen Ziziphi Spinosae water extract
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Lee Sang-Won
Lyu Yeoung-Su
Kim Tae-Heon
Kim Dae-Hyun
Lee Seong-Geun
Lee Key-Sang
Yun Jong-Hyun
Kang Hyung-Won
Kim Jin-Woo
Jung Eun-Young
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Abstract
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Objective: The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H202-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12.
Methods: The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay.
Results and Conclusions: 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions. 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer’s disease.
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KEYWORD
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Semen Ziziphi Spinosae(SZS), pc 12, Antioxidant, Anti-Alzheimer, MTT assay, LDH assay
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